A team of scholars from Mainz University Medical Center and Goethe University Frankfurtunknown say they have discovered a new harmful effect of neonicotinoids in low and field-relevant concentrations.
The effect, they believe, is to reduce the concentration of acetylcholine, a signaling molecule relevant for the development of the honeybee larvae, in the royal jelly/larval food secreted by nurse bees. At higher doses, they found that neonicotinoids also damage the so-called microchannels of the royal jelly gland in which acetylcholine is produced. Comparable to neonicotinoids, it stimulates the nicotinic acetylcholine receptors that are also present in this gland.
In order to find an effect of neonicotinoids on the acetylcholine content in the jelly in more detail, bee colonies were exposed to various concentrations of neonicotinoids in flight tunnels (clothianidin: 1, 10 and 100 µg/kg glucose solution; thiacloprid 200 and 8800 µg/kg). “This exposure led to a reduction in the acetylcholine content of the jelly. Thus we were able to demonstrate that the field-relevant dose of the neonicotinoid agent thiacloprid (200 µg/kg) significantly reduces acetylcholine content by 50 percent. On exposure to higher doses, we were even able to verify that acetylcholine content can be reduced by 75 percent. Exposure of the bees with the higher doses results in serious damage to the microchannels and secretory cells of the jelly gland,” said Professor Ignatz Wessler of the Institute of Pathology at the University Medical Center of Johannes Gutenberg University Mainz. “Our research results thus confirm that the neonicotinoids can jeopardize the normal development of honeybee larvae.”
Citation: Wessler, I., Gärtner H.-A., Michel-Schmidt R., Brochhausen C., Schmitz L., Anspach L., Grünewald B., Kirkpatrick C.-J., Honeybees Produce Millimolar Concentrations of Non-neuronal Acetylcholine for Breeding: Possible Adverse Effects of Neonicotinoids. PLOSONE, 10 June 2016, DOI:10.1371/journal.pone.0156886